REVERSAL OF COMPLEX VANADIUM (VO3 -) AND TUNGSTEN (WO2- 4) RESISTENCE IN TOBACCO CELL LINES
DOI:
https://doi.org/10.32782/1998-6475.2024.56.7-11Keywords:
tobacco, cell lines, resistance, vanadium, tungsten, nitrate reductase (NR)Abstract
Heavy metal ions have a complex effect on living organisms. Resistance to them should cause significant changes that must be genetically determined. When selecting resistant cellular variants, cells that are characterised by stable growth in the presence of a constant stress factor, it is necessary to investigate the cause of resistance. Obviously, cross-resistance is possible when genetic and epigenetic changes are combined. This is particularly relevant because epigenetic changes are caused by mechanisms that normally operate during cellular differentiation. The study of cell lines resistant to PMI has shown that cell culture in general and cell selection in particular has not exhausted its potential. As a method of studying the fundamental mechanisms of cell functioning under normal and stress conditions, it is difficult to surpass. As a way to produce resistant plants, it is a promising biotechnological approach. As a research ideology, it is aimed at promoting environmental safety in experimental and production activities. Vanadium- and tungsten-resistant tobacco cell lines were cultured on selective media containing toxic concentrations of alternative oxyanions. The clones selected as a result of cellular selection possessed complex resistance to tungstate and vanadate. The resistance of the cells is associated with the selection of resistant cells, possibly by vanadium-dependent nitrate reductase (NR).
References
AIHEMAITI, A., GAO, Y., MENG, Y., CHEN, X., LIU, J., XIANG, H., XU, Y., JIANG, J. (2020) Review of plantvanadium physiological interactions, bioaccumulation, and bioremediation of vanadium-contaminated sites. Science of the Total Environment, 712, 135637. DOI: 10.1016/j.scitotenv.2019.135637.
ANGULO-BEZARANO, P.I., PUENTE-RIVERA, J., CRUZ-ORTEZA, R. (2021) Metal and metalloid toxicity in plants: an overview on molecular aspects. Planta, 10(4), 635. DOI: 10.3390/plants/0040635.
BALASUBRANIAM, T., SHEN, G., ESMAEILI, N., ZHANG, H. (2023) Plants’ response mechanisms to salinity stress. Plants, 12, 2253, 1–22. DOI: 10.3390/plants12122253.
BERGER, A., BOSCARI, A., ARAÚJO, N.H., MANCOURT, M., HANCHI, M., BERNILLON, S., KOLIN, O., PUPPO, A., BROUQUISSE, R. (2020) Plant nitrate reductases regulate nitric oxide production and nitrogen – fixing metabolism during the Medicago truncatula – Sinorhizobium melloti simbiosis. Frontiers in Plant Science, 11. DOI: 10.3389/fpls.2020.01313.
El-FAYOUMY, E.A., SHANAB, S.M., HASSAN, O.M.A., SHALABY, E.A. (2023) Enhancement of active ingredients and biological actives of Nostoc linkia biomass cultivated under modified BGII0 medium composition. Biomass Conversion and Biorefinely, 13, 6049–6066. DOI: 10.1007/s13399-021-01509-7.
FU, Y.-F., ZHANG, Z.-W., YUAN, S. (2018) Putative connections betwen nitrate reductase S – nitro solation and NO synthersis under pathogen attacks and abiotic stress. Frontiers in Plant Physiology, 9. DOI: 10.3389/2018.00474.
GAMBORG, O.L., MILLER, R.A., OJIMA, K. (1968) Nutrient requirements of suspension culture of soybean root cells. Experimental Cell Research, 50(1), 151–158.
HAO, L., WANG, X., SHI, J., LI, L., HAO, X. (2023) Vanadium (V) bio-detoxification based on washing water of vise as microbial and carbon sources. Frontiers in Environmental Science, 11. DOI: fenvs.2023.1096845.
HARJA, M., CIOCINTA, R.C., ONDRASEK, G., BUCUR, D., DIRJA, M. (2023) Accumulation of heavy metal ions from urban soil in spontaneous flora. Journal Water, 15(4), 768–775. DOI: 10.3390/w1504768.
HE, M., HE, C.-Q., DING, N.-Z. (2018) Abiotic stresses: general defeaces for engineering multistress tolerance. Frontiers in Plant Science, 9, 1771. DOI: 10.3389/fpls2018.01771.
KAUFHOLDT, D., BAILLIE, C.-K., MÜNEM, R., MENDEL, R.R., HÄNSCH R. (2017) The molybdenum cofactor biosynthesis network: in vivo protein – protein interactions of an active associated multi – protein complex. Frontiers in Plant Science, 8, 1946–1957. DOI: 10.3389/fpls.2017.01946.
KOYAMA, L.A., TERAI, M., TOKUCHI, N. (2020) Nitrate reductase actives in plants from different ecological and taxonomic groups grown in Japan. Ecological Research, 35(5), 708–712. DOI: 10.1111/1440-1703.12083.
LOHANI, N., SING, M., BHALLA, P (2022) Biological parts for engineering abiotic stress tolerance stress in plants. BioDesing Research, 41. DOI: 10.34133/2022/9819314.
MACÄRIO, I.P.E., VELOSO, T., ROMÃO J., CONCALVES, J.M., PEVERA, J.L., DUARTE, I.F., VENTURA, S.P.M. (2022) Metabolic composition of the cyanobacterium Nostoc muscorus as a function of culture time: A1HNMR metabolomics study. Algal Research, 66, 102792. DOI: 10.1016/j.algal.2022.102792.
MALIGA, P. (2003) Isolation and characterization of mutants in plant cell culture. Annual Review of Plant Ohysiology and Plant Molecular Biology, 35(1), 519–542. DOI: 10.1146/annurev.pp.35.060184.002511.
MORKUNAS, J., WOŹNIAK, K., MAI, V.C., RUCIŃSKASOBKOWIAK, K., JEANDER, P. (2018) The role of heavy metal in plant response to biotic stress. Molecules, 23(9), 2320. DOI: 10.3390/molecules23092320.
OCHOA-VILLARREAL, M., HOWAT, S., HONG, S., JANG, M.O., JIN, Y.-W., LEE, E.-K., LOAKE, G.J. (2016) Plant cell culture strategies for the production of natural produced. BMB Reports, 49(3), 149–158. DOI: 10.5483/BMBRep.2016.49.3.264.
PASTERNAK, T.P., STEINMACHER, D. (2024) Plant growth regulation in cell and tissue culture in vitro. Plants, 13(2), 327. DOI: 10.3390/plants13020327.
SERGEEVA, L.E., MYKHALSKA, S.I. (2019) Cell selection with heavy metal ions for obtaining salt tolerant plant cell cultures. Plant Physiology and Genetics, 51(4), 315–323. DOI: 10.15407/frg2019/04/315.
SERGEVA, L.E. (2017) Nitrate reductase activity in biotechnology tobacco plants under enzyme inhibitors action. Plant Physiology and Genetics, 49(2), 129–133. DOI: 10.15407/frg2017.02.129.
SINH, H.N., CGACRAVARTY, D., SRINISAVA, A.K. (1993) Vanadium requiments for growth on N2 or netrate as nitrogen source in tungsten resistant mutant of the cyanobacterium Nostoс muscorum. Journal of Basic Microbiology, 33(3), 201–205.
ZHANG, Y., XU, L., LI, R., GE, Y., LI, Y., LI, R. (2023) Plants’ Response to abiotic stress: mechanisms and strategies. International Journal of Molecular Sciences, 24(13), 10915. DOI: 10.3390/ijms241310915.