Oxidative stress in experimental vaginitis and its correction
DOI:
https://doi.org/10.32782/2077-6594/2023.2/15Keywords:
oxidative stress, vaginitis, vaginal tablets “Mycogynax”, “Suppositories with sea buckthorn oil”Abstract
Purpose is to compare the pharmacological effectiveness and influence of vaginal tablets “Mycogynax” and “Suppositories with sea buckthorn oil” on the development of oxidative stress in experimental vaginitis. Material and methods. The study was conducted on 24 white outbred sexually mature female rats. The animals were divided into 4 groups: 1st – control (intact rats); 2-nd – experimental vaginitis; 3rd – experimental vaginitis + “Mycogynax” vaginal tablets; 4th – experimental vaginitis + “Suppositories with sea buckthorn oil”. The therapeutic efficacy was studied on the induced vaginitis model, the treatment lasted for 7 days, after which the concentration of TBK-active products, diene conjugates, activity of superoxide dismutase, catalase, and ceruloplasmin content were determined in the blood of animals. Results. In the course of the experiment, it was established that against the background of induced vaginitis in rats, deterioration of the state of the POL-AOS system was observed, which was evidenced by a significant increase in TBK-active products and DC compared to the control group. With an increase in POL, the activity of the enzymatic link of AOS decreased, namely, the activity of SOD decreased by 46.4 % and catalase by 37.1 % (p < 0.05) compared to the intact group. So, in the conditions of experimental vaginitis, changes in the pro-oxidant-antioxidant balance in the blood were characterized by the development of oxidative stress. We have established that when using “Mycogynax” tablets, there was an increase in the activity of the enzyme link of AOS, in particular, the activity of SOD increased by 127.7 % (р < 0.05), and catalase in the blood plasma – by 55.6 % (р < 0 .05), and when using “Suppositories with sea buckthorn oil” the level of SOD increased by 159.9 % (p < 0.05), and catalase – by 82.1 % (p < 0.05), compared to the 2nd experimental groups The obtained data indicate the inhibition of oxidative stress in the blood of animals with experimental vaginitis when using pharmacotherapy, and the use of suppositories with sea buckthorn oil shows a more effective therapeutic effect. Conclusions. It has been established that for the treatment of vaginitis, the use of the herbal preparation “Suppositories with sea buckthorn oil” is more effective from the point of view of biochemical processes in the body.
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