Effectiveness of cell-free cryopreserved biological agents in autoimmune arthritis based on hematological changes
DOI:
https://doi.org/10.32782/2077-6594/2024.2/09Keywords:
placenta cryoextract, spleen cryoextract, conditioned medium of mesenchymal stem cells, prostaglandins, thromboxane, leukotrienes, autoimmune diseases, anemia of chronic inflammation, leukocytesAbstract
The aim of the work is to characterize the effects of cryoextract of the placenta (СEP), cryoextract of the spleen (СES) and MSC mesenchymal stem cells conditioned media (CS-MSC) on hematological parameters of rats with adjuvant arthritis (AA). Research materials and methods. Experimental studies were conducted on 42 male rats weighing 200–220 g. AA was modeled by administration of complete Freund's adjuvant (CFA). AA treatment was carried out from 14 to 28 days. CEP, CES and CS-MSC were injected on days 14, 17, 20, 23 and 26, respectively. Hematological studies were performed on the 28th day of the experiment. Results and their discussion. Against the background of the administration of cryoextracts, an increase in the content of erythrocytes was also noted, but to a lesser extent – against the background of the administration of CEP, the content of erythrocytes increased by 45.5% (р˂0.05), and against the background of the introduction of CES – it increased by 65.6% (р˂0.001) relative to the indicators of untreated rats with AA. The assessment of the intensity of the inflammatory process against the background of the use of the investigated cell-free biological agents showed that the most pronounced anti-inflammatory activity was shown by the introduction of CM-MSC – the leukocyte content was 9.9±0.63 × 109/l, and the erythrocyte sedimentation rate was 5.7± 0.64 mm/h, which was 53.4% and 69.0% lower, respectively, than similar indicators in animals of the control group. Conclusions. According to the amount of reduction in the content of leukocytes in rats with AA on the 28th day of the experiment, the studied biological agents can be arranged in the following sequence: CM-MSC (53.4%) ˃ CEP (39.9%) ˃ CES (32.4%). According to the magnitude of the decrease in erythrocyte sedimentation rate on the 28th day of the experiment relative to the indicators of rats in the control group, the cell-free cryopreserved agents administered to rats with AA can be arranged in the following order: CM-MSC (69.0%) ˃ CES (52.7%) ˃ CEP (19.6%).
References
Shi J, Chi S, Xue J, Yang J, Li F, Liu X. Emerging Role and Therapeutic Implication of Wnt Signaling Pathways in Autoimmune Diseases. J Immunol Res. 2016;2016:9392132. https://doi.org/10.1155/2016/9392132
Cici D, Corrado A, Rotondo C, Cantatore FP. Wnt Signaling and Biological Therapy in Rheumatoid Arthritis and Spondyloarthritis. Int J Mol Sci. 2019;20(22):5552. https://doi.org/10.3390/ijms20225552
Sarsenova M, Issabekova A, Abisheva S, Rutskaya-Moroshan K, Ogay V, Saparov A. Mesenchymal Stem Cell-Based Therapy for Rheumatoid Arthritis. Int J Mol Sci. 2021;22(21):11592. https://doi.org/10.3390/ijms222111592
Aletaha D, Smolen JS. Diagnosis and Management of Rheumatoid Arthritis: A Review. JAMA. 2018;320(13):1360-1372. https://doi.org/10.1001/jama.2018.13103
Smolen JS, Aletaha D, Barton A, Burmester GR, Emery P, Firestein GS, Kavanaugh A, McInnes IB, Solomon DH, Strand V, Yamamoto K. Rheumatoid arthritis. Nat Rev Dis Primers. 2018;4:18001. https://doi.org/10.1038/nrdp.2018.1
Lopez-Santalla M, Bueren JA, Garin MI. Mesenchymal stem/stromal cell-based therapy for the treatment of rheumatoid arthritis: An update on preclinical studies. EBioMedicine. 2021;69:103427. https://doi.org/10.1016/j.ebiom.2021.103427
Hladkykh FV. Current understanding of the immunological basis of rheumatoid arthritis: from post-translational modification of proteins to the use of disease-modifying antirheumatic drugs. Eastern Ukrainian medical journal 2023;11(4):326-336. https://doi.org/10.21272/eumj.2023;11(4):326-336
Stefanov OV, ed. Preclinical studies of medicinal products: methodical recommendations. Kyiv: Avicenna; 2001. 527 p.
Hladkykh FV. Nonsteroidal anti-inflammatory drugs: therapeutic and undesirable effects, ways of their optimization. Vinnytsia: Tvoty; 2022. 216 p. https://doi.org/10.46879/2022.1
Shepitko VI. Structural and functional indicators of the cryopreserved liver and the effect of its transplantation on the morphofunctional state of a number of internal organs: dissertation. Doctor of Medicine: special. 14.01.35 – Cryomedicine, Kharkiv, 2004. 326 p. https://nrat.ukrintei.ua/searchdoc/0504U000610/
Bespalova IG. Peptide composition and biological action of extracts of cryopreserved pig spleen fragments and piglet skin: thesis. biol. n.: in specialty 03.00.19 – Cryobiology, Kharkiv, 2016. 162 p. https://nrat.ukrintei.ua/searchdoc/0416U004539/
Golubinskaya PA, Sarycheva MV, Dolzhikov AA, Bondarev VP, Stefanova MS, Soldatov VO, Nadezhdin SV, Korokin MV, et al. Application of multipotent mesenchymal stem cell secretome in the treatment of adjuvant arthritis and contact-allergic dermatitis in animal models. Pharmacy & Pharmacology. 2020;8(6):416-425. https://doi.org/10.19163/2307-9266-2020-8-6-416-425
Globa VYu. Use of cryopreserved cell cultures and neurotrophic factors in experimental infravesical obstruction. Thesis in specialty 222 – Medicine, Kharkiv, 2021. 156 p. https://nrat.ukrintei.ua/searchdoc/0821U100913/
Zar JH. Biostatistical analysis (5 ed.). Prentice-Hall, Englewood. 2014; 960 р.
Swann JW, Koneva LA, Regan-Komito D, Sansom SN, Powrie F, Griseri T. IL-33 promotes anemia during chronic inflammation by inhibiting differentiation of erythroid progenitors. J Exp Med. 2020;217(9):e20200164. https://doi.org/10.1084/jem.20200164
