Antimicrobial activity of methylene blue and LED radiation in vitro and in vivo
DOI:
https://doi.org/10.32782/2786-7684/2024-2-11Keywords:
low-intensity radiation, Streptococcus mutans, chronic generalized periodontitis, antimicrobial photodynamic therapyAbstract
Introduction. Antibiotics play a key role in the treatment and control of bacterial infections. The emergence and spread of antimicrobial resistance increases the morbidity and mortality rate of diseases and causes significant economic losses. One of the ways to overcome this global problem is the use of photochemical methods, in particular antimicrobial photodynamic therapy. The purpose of the work was to determine the combined effect of methylene blue photosensitizer and red spectrum LED radiation on microorganisms isolated from patients with chronic generalized periodontitis in experimental and experimental-clinical studies. Materials and methods. The combined effect of a 0.1% aqueous solution of methylene blue and LED radiation of the red-infrared range on the growth intensity of Streptococcus mutans in vitro, as well as the effect of these factors on the microbiome of the periodontal pockets of patients with chronic generalized periodontitis, was studied. Results and discussion. It was established that the combined effect of the photosensitizer and low-intensity radiation causes a significant antimicrobial effect on the studied strain, which significantly exceeds the effect of the impact of these factors separately. A bactericidal effect on periodontal pockets microflora was noted when using the proposed method in clinical studies. Conclusions. Taking into account several advantages of using photodynamic therapy – activity against a wide range of pathogens, the possibility of carrying out procedures in hard-to-reach places, low invasiveness, and the absence of side effects, this method can be used in the treatment of chronic generalized periodontitis and other pathologies of the oral cavity caused by pathogenic and opportunistic microorganisms.
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