Efficacy of bacteriophages against isolates of complex gunshot wounds in in vitro experiments
DOI:
https://doi.org/10.32782/2786-7684/2024-2-16Keywords:
bacteriophages, antibiotic resistance, phage sensitivity, in vitro, phage-antibiotic synergyAbstract
A significant increase in antibiotic resistance, high mortality and disability rates due to the formation of multidrug-resistant and panresistant infections both in monoculture and in associations necessitates the search for new tools and methodological techniques for the treatment of complicated persistent infections, especially in the context of complex wound infections. In the search for alternative antimicrobial agents, bacteriophages have once again begun to attract the attention of researchers and clinical surgeons. The aim of the study was to investigate the sensitivity of isolates isolated from the wound surfaces of gunshot wounds to bacteriophages. The biological material was collected at the Central City Clinical Hospital. For this purpose, a swab was taken from the wound surface using a sterile transport system, FLmedical (Italy). The antimicrobial activity was studied in the microbiological laboratory of the Research and Educational Centre ‘Microbiology and Biotechnology’. The following clinical isolates were used for the study: Escherichia coli, Klebsiella spp., Staphylococcus aureus and Pseudomonas spp. The bacteriophage isolates were provided by Neoprobiocare-Ukraine LLC. It was found that the bacteriophage was most effective against Staphylococcus aureus genus bacteria. The susceptibility tests of clinical isolates of S. aureus showed that their phage susceptibility was 81.8%. Among the studied Klebsiella spp. strains, 60% were resistant to a specific bacteriophage. The studied isolates of Escherichia coli and Pseudomonas spp. showed low sensitivity to bacteriophages (33.3% and 16.7%, respectively). The data obtained indicate the potential effectiveness and good prospects of phage-based therapy in the treatment of wound infections. The strain-specific susceptibility to bacteriophages indicates the feasibility of a patient-centred approach and determination of the isolate’s susceptibility to bacteriophage before starting treatment.
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