Justification for the use of exogenous prophylactic compositions to control biofilm formation on tooth enamel in children

Authors

DOI:

https://doi.org/10.32782/2077-6594/2025.2/03

Keywords:

caries, temporary teeth, permanent teeth, biofilm, remineralization, Quorum Sensing, statistical correlations

Abstract

Purpose: to assess the effect of drugs for remineralizing caries prevention on biofilm adhesion to tooth enamel to prevent enamel demineralization in children.Material and methods. The study included 521 individuals (163 preschoolers and 183 younger schoolchildren in the leading group and 175 children in the control group). Patients in the leading group were given exogenous caries prophylaxis with Bifluorid 10 varnish and Remin Pro cream (VOCO, Germany). The state of the oral hygiene index was assessed using the Fedorov-Volodkina index. Descriptive statistical analysis methods and Spearman's correlation coefficient [r] were used to assess the relationship between the studied parameters. The significance level was set at 5%.Results. The most effective method of preventing biofilm formation on the enamel surface, especially on primary teeth, which are rich in acid-forming streptococci, is to prevent bacteria from adhering to the enamel surface. Such properties are presented among the preparations used for teeth enamel remineralization, as confirmed by the significant improvement of the hygiene index in children in the leading group, particularly for the good [0; 42%; p <0.001] and satisfactory index [6%; 51%; p <0.001].Conclusions. According to individual clinical findings, Remin Pro and Bifluorid 10 effectively prevent biofilm adhesion on enamel surfaces and stop the demineralization of enamel in permanent and temporary teeth among children. It is also an effective method of primary caries prophylaxis.

References

Klitynska O, Kostenko Y, Gurando V. Determination of criteria for early caries diagnosis in children of different ethnic groups living in areas with biogeochemical deficiency of fluoride and iodine. Journal of Stomatology. 2017;1(70):51–56.

Klitynska OV, Gasyuk NV, Kostenko YY, Viacheslav R Gurando. Statistical model of caries formation and progression in preschool and early school-age children living in areas with biogeochemical deficiency of fluoride and iodine. Journal of Stomatology. 2017;6(70):674–678. doi: 10.5604/01.3001.0010.7725.

Damle SG. Competence and transformation of oral Streptococcus sobrinus in dental caries. Contemporary Clinical Dentistry. 2018;9 (Suppl 2):195–S196. doi: 10.4103/ccd.ccd_689_18.

Klitynska OV, Hasiuk NV, Hasiuk PV, Vasko AA, Gurando VR, Zorivchak TI, Stishkovsky AV. Statistical analysis of criteria for efficiency of filling of permanent teeth in children. Acta Stomatologica Naissi. Dec 2021;37(84):2232–2240.

Pešić Stanković J, Kostić M, Igić M, Đorđević V. Biofilm formation on dental materials. Acta Stomatol Naissi. 2018;34(77):1821–1831.

Kostić M, Pešić Stanković J, Petković D, Igić M, Vasić Bradić M, Glogorijević N. Comparison of surface roughness of dental materials as an adhesion factor of oral biofilm. Acta Stomatol Naissi. 2019;35(80):1956–1969.

Coulter J, Jakubovics NS, Preshaw PM, German MJ. An in vitro model to assess effects of a desensitizing agent on bacterial biofilm formation. Acta Biomater Odontol Scand. 2018;5(1):1–8. doi:10.1080/23337931.2018.1544847.

Jeong D., Kim D.H., Song K.Y., Seo K.H. Antimicrobial and anti-biofilm activities of Lactobacillus kefiranofaciens DD2 against oral pathogens. Journal of Oral Microbiology. 2018;10(1):1472985. doi:10.1080/20002297.2018.1472985.

Jingarwar M.M. “Minimal intervention dentistry - a new frontier in clinical dentistry.” Journal of Clinical and Diagnostic Research. 2014;8(7):ZE04-8. doi:10.7860/JCDR/2014/9128.4583.

Pinar Erdem A., Sepet E., Kulekci G., Trosola S.C., Guven Y. Effects of two fluoride varnishes and one fluoride/chlorhexidine varnish on Streptococcus mutans and Streptococcus sobrinus biofilm formation in vitro. International Journal of Medical Sciences. 2012;9(2):129–136. doi:10.7150/ijms.3637.

Nealson K.H., Platt T., Hastings J.W. Cellular control of the synthesis and activity of the bacterial luminescence system. Journal of Bacteriology. 1970;104:313-322.

Scalioni F., Carrada C., Machado F. Salivary density of Streptococcus mutans and Streptococcus sobrinus and dental caries in children and adolescents with Down syndrome. Journal of Applied Oral Science. 2017;25(3):250–257. doi:10.1590/1678-7757-2016-0241.

Diggle S.P., Winzer K., Lazdunski A., Williams P., Camara M. Advancing the quorum in Pseudomonas aeruginosa: MvaT and the regulation of N-acylhomoserine lactone production and virulence gene expression. Journal of Bacteriology. 2002;184(10):2576–2586.

Zaura E., Twetman S. Critical appraisal of oral pre- and probiotics for caries prevention and care. Caries Res. 2019 Apr 4;53(4):375-387. doi: 10.1159/000499037.

Barbour A., He R.Y., Gong S.G., Lévesque C.M. Complete genome sequence of Streptococcus mutans strain LAB761, which harbors several bacteriocin loci, isolated from a caries-active child in Canada. Microbiol Resour Announc. 2019;8(2):e01483-18. doi: 10.1128/MRA.01483-18.

Keller M.K., Kressirer C.A., Belstrøm D., Twetman S., Tanner A.C.R. Oral microbial profiles of individuals with different levels of sugar intake. J Oral Microbiol. 2017;9(1):1355207. doi: 10.1080/20002297.2017.1355207.

Parsek M.R., Val D.L., Hanzelka B.L. Acyl homoserine lactone quorum-sensing signal generation. Proc. Natl. Acad. Sci. USA. 1999;96(8):4360-5. doi: 10.1073/pnas.96.8.4360.

Farkash Y., Feldman M., Ginsburg I., Steinberg D., Shalish M. Polyphenols inhibit Candida albicans and Streptococcus mutans biofilm formation. Dent J (Basel). 2019 Apr 11;7(2):42. doi: 10.3390/dj7020042.

Downloads

Published

2025-06-30

How to Cite

Клітинська, О., Мартиць, Ю., Гурандо, В., Одноралов, А., & Хащевацький, А. (2025). Justification for the use of exogenous prophylactic compositions to control biofilm formation on tooth enamel in children. Ukraine. Nation’s Health, (2), 18–22. https://doi.org/10.32782/2077-6594/2025.2/03

Issue

Section

Public health