Study of cumulative properties of diclofenac sodium and features of biological action in a sub-acute experiment

Abstract

Purpose. To determine and investigate the structural-metabolic and functional status, cumulative properties of diclofenac sodium in subacute experiments on warm-blooded animals. Materials and methods. The study of the cumulative properties, hematological and biochemical parameters of the blood of non-linear rats and white mice was carried out by generally accepted methods The calculation and analysis of the obtained data was carried out using generally accepted methods of statistical processing of the results of medical and biological research (determination of arithmetic mean values of the studied indicators, standard error, square deviation) with the calculation of the Student's t-criterion. The results. Based on the results of daily intragastric and intranasal administration of one-twentieth the DL50 of diclofenac sodium, accumulation coefficients were determined (0.94 for white mice and 0.96 for white male rats, respectively). Quantitative and qualitative changes in hematological indicators may indicate insufficient oxygen transport function of erythrocytes, hypoxic manifestations in the body, slowing down of redox reactions, strengthening of immune response and reactivity. There is a tendency to decrease protein on day 10. The obtained results showed that the studied substance (diclofenac sodium) can cause a violation of indicators of metabolic processes in organs and tissues. Conclusions. The established parameters of the subacute experiment and the determination of the cumulative properties of diclofenac sodium testify to the supercumulative properties of the substance under study, since Kk < 1. It was established that diclofenac sodium belongs to highly toxic substances (toxicity class 2).