INFLUENCE OF DIETHYL ESTER OF 5-ALKYLAMINO-2-{N-[N-BENZOYL- (4-METHYLBENZYLIDENE)GLYCYL]AMINOMETHYL}-1,3-OXAZOL-4-YL PHOSPHONIC ACID ON ERYTHROCYTE MEMBRANE RESISTANCE IN ETHANOL-INTOXICATED RATS

Abstract

Introduction. Alcohol intoxication is accompanied by disturbances in the structural and functional organization of erythrocyte membranes. This highlights the need to identify new biologically active compounds with membrane‑stabilizing and membrane‑protective properties. Particular scientific interest is focused on derivatives of 1,3-oxazole and phosphonic acid, for which a wide range of biological activities has been described. Нowever, their effect on erythrocyte resistance has not been sufficiently investigated. Materials and Methods. The study was conducted on sexually mature female white rats, which were administered diethyl ester of 5‑alkylamino‑2‑{N‑[N‑benzoyl‑(4‑methylbenzylidene)glycyl]aminomethyl}‑1,3‑oxazol‑4‑ylphosphonic acid (OVP‑1) at a dose of 25 mg/kg prior to the introduction of 50% ethanol (5 ml/kg). The functional state of erythrocyte membranes was assessed by indicators of osmotic, acid, and peroxide resistance. Statistical analysis was performed using IBM SPSS Statistics 19.0, with significance determined at P < 0.05. Results. Intraperitoneal administration of OVP‑1 resulted in a 38.8% decrease in erythrocyte hemolysis in 0.6% NaCl solution, decreased the peak of the acid erythrogram by 14.28%, and lowered the peroxide resistance index by 1.2‑fold compared with alcoholized animals. In addition, under the influence of OVP-1, the time to complete erythrocyte hemolysis in an acidic medium was restored to the level observed in intact animals. Conclusions. OVP-1 exhibits membrane-stabilizing and membrane-protective properties under conditions of alcohol intoxication, suggesting its potential as a promising biologically active compound for correcting disturbances in the structural and functional state of cellular membranes and the rheological properties of blood.