ANALYSIS OF SEED GENERATIONS OF BIOTECHNOLOGICAL WHEAT PLANTS AN ADDITIONAL COPY OF THE GENE ORGYTIN-δ-AMINOTRANSFERASE OF ALFALFA
DOI:
https://doi.org/10.32782/1998-6475.2025.58.11Keywords:
proline, Triticum aestivum L., osmotic stress, drought, productivity, yield, resistance, transgenic plants.Abstract
Cultivation of biotechnological winter wheat progeny under water deficit and salinity conditions allowed to analyse the level of free proline and to relate it to grain productivity. Under stressful conditions, the superiority of free proline content in the vegetative organs of genetically modified wheat compared to the original forms was noted.It was shown that the main indicators of the yield structure of the seed generation of wheat with a functional transgenesignificantly exceeded the control variants under normal and stress conditions. The object of the study was T1–T4 variants of winter wheat, genotype UK-209, UK 322/17. The aim of this study was to determine the tolerance to water deficit of seed generations T1–T4 of genetically modified wheat with partially suppressed expression of the proline dehydrogenase (ProDH) gene based on physiological and biochemical parameters and economic characteristics of plants. We used the following research methods: determination of yield structure parameters and biochemical methods for determining L-proline (Pro).The level of Pro- and analysed elements of productivity in the progeny of transgenic plants and their original forms under normal and insufficient water supply was investigated. The winter wheat plants of T1-T4 generations of genotype UK 322/17, UK 209 h were studied for resistance to water and salt stress. The responses to short-term salinity and water deficit associated with the accumulation of free proline, as well as the nature of recovery fromstress were analysed.
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