Inhibition of the growth of gram-negative bacteria by anthocyanins of berries fruits
DOI:
https://doi.org/10.24144/1998-6475.2019.46-47.42-47Keywords:
agar diffusion method, antimicrobial activity, bacterial strains, plant extracts.Abstract
Plants contain biologically active compounds useful in the prevention and therapy of different types of diseases. Polyphenol compounds, especially anthocyanins are the subject of extensive research in view of their biological activities. Anthocyanins are rich in their antimicrobial effects especially. The aim of this study was to assess the antimicrobial effect of extracts rich on anthocyanins prepared from the berry fruit (Vaccinium myrtillus L., Aronia melanocarpa L. and Sambucus nigra L.) by acetone and ethanol extraction. The effect of the extracts on the growth of Gram-negative bacteria (Escherichia coli, Salmonella enterica ser. Typhimurium, Pseudomonas aeruginosa, Enterobacter aerogenes) using the plate diffusion method was evaluated. Based on the results it can be stated that the anthocyanins isolated from berries reached some degree of antimicrobial activity. The bacterial strain of E. coli was the most sensitive to the action of tested extracts. The best antimicrobial activity was found in Vaccinium myrtillus ethanol extracts against gram-negative bacteria. Ethanol extracts were more effective compared to the acetone extracts. In conclusion, the berry fruits important in human nutrition as an important group of natural antioxidants. It can also acts as preventive and therapeutic agents for various bacterial infections.
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