ANTIBIOTIC SUSCEPTIBILITY OF FUNGAL AND BACTERIAL COMMUNITIES FROM INFECTED GUNSHOT WOUND SURFACES

Abstract

The publication presents part of our research on antibiotic sensitivity of microbial communities in purulent wounds of wounded soldiers treated in medical institutions in Transcarpathia (Zakarpatska Oblast, Ukraine). In 32.2% of the samples obtained from the purulent wounds, associations of microscopic fungi Candida glabrata with a different spectrum of opportunistic bacteria were identified. In no case was Candida glabrata detected as a monoculture; the fungi were found only in association with bacteria. In total, our studies identified four associations of Candida glabrata with bacteria. Three of these associations were found to be two-component: Candida glabrata + Staphylococcus aureus; Candida glabrata + Pseudomonas spp. and Candida glabrata + Klebsiella spp.; one was three-component: Candida glabrata + Staphylococcus aureus + Enterobacter spp. The study of sensitivity to 30 antibiotics and 5 antifungal drugs used in medical institutions showed a significant level of resistance of the isolated microorganisms to antibiotics. Groupings involving fungi and bacteria exhibit complex resistance and pose a problem because the elimination or reduction of the persistence of one of the associates does not solve the problem of infection, but merely leads to the transformation of the grouping. In such circumstances, research into the antibiotic sensitivity of all microbial associates isolated from the inflammatory site and selection of the optimal treatment protocol becomes especially important. Along with the search for the dosing schedule of new antibiotics and antifungal drugs, it is necessary to look for alternative biologically active compounds with antimicrobial properties.