Analysis of the antiseptic effects on root canal microbiota in acute pulpitis of primary teeth

Abstract

The aim of the study. To substantiate the choice of antiseptic drugs considering the sensitivity of the pathogenic microbiota of root canals in acute forms of pulpitis of primary teeth and to determine the minimum inhibitory concentrations of antiseptics against clinical and typical microorganisms.Materials and methods. The study analyzes the effectiveness of antiseptics on the microbiota of root canals in acute pulpitis of primary teeth. Various antiseptic solutions, including sodium hypochlorite, chlorhexidine, iodine, and creosote, were tested against clinical and typical strains of bacteria and fungi. For the study, typical museum cultures and clinical cultures isolated from root canals in pulpitis of primary teeth were used: microscopic fungi C. albicans, S. aureus, S. epidermidis, E. coli, K. Oxitoca, S. viridans, S. pyogenes, E. faecalis.The antibacterial and antimycotic effects of the studied compounds were also evaluated by the minimum inhibitory concentration (MIC).Results. Results showed that chlorhexidine bigluconate 2%, iodine 5%, and creosote 0.01% had the highest antimicrobial activity, while sodium hypochlorite was effective against gram-negative bacteria. Some antiseptics exhibited limited or no effect on specific pathogens. The study highlights the need for optimized antiseptic concentrations to ensure effective disinfection and improve endodontic treatment outcomes in pediatric dentistry, helping prevent reinfections and enhance long-term success. Against E. coli and K. oxitoca, the highest activity was shown by chlorhexidine bigluconate 2%, iodine 5% and creosote 0.01%. Sodium hydrochlorit 3% and 5% had high activity against typical and clinical strains of E. coli. Against K. oxitoca, 5% Sodium hydrochlorit had moderate antimicrobial activity and 3% Sodium hydrochlorit had low activity. Furacilin solution 0.06%, chlorophyllipt 1%, the drug "Hepilor" (choline salicylate 5%) and chlorhexidine 5% had no antimicrobial effect on gram-negative microorganisms taken into the experiment. These findings support evidence-based antiseptic selection for safe and efficient root canal disinfection, reducing complications and improving patient care in clinical practice.