Zinc and magnesium content in pancreatic islets of animals with different experimental models of diabetes mellitus
DOI:
https://doi.org/10.32782/2786-7684/2025-2-20Keywords:
diabetogenic substances, correlations, intracellular metals, pancreatic isletsAbstract
Introduction. It is known that the implementation of the mechanisms of diabetes development is based on significant disturbances in the body's metabolic processes, including the metabolism of mineral substances. In our opinion, studies of zinc and magnesium, which play an important role in the activity of the insular apparatus and the regulation of carbohydrate metabolism, deserve special attention.The aim of the study. To study the changes in the content of zinc and magnesium in the pancreatic islets of mice and rats upon administration of various diabetogenic substances.Materials and methods. White outbred mice and rats were used in the experiments. The development of methods for determining zinc and magnesium in islet β cells in our laboratory allowed us to study the content of these metals in animals using various experimental models of diabetes mellitus.Results of the study and their discussion. It was found that after the administration of alloxan in mice, alloxan and streptozotocin in rats, a significant deficiency of zinc and magnesium in β-cells was observed. Less pronounced changes were observed in mice with streptozotocin- induced diabetes. A slight deficiency of metals was observed in mice with dithizone diabetes and diabetes caused by 8-TSQ, and in rats – also with dithizone. With an increase in the severity of alloxan diabetes, a gradual decrease in the content of zinc and magnesium in the pancreatic islets of animals was observed.Conclusions. 1. Zinc deficiency in insulin-producing cells of mice increased from 28% after administration of 8-TSQ to 69% (P<0,001) – alloxan, and in rats – 19% (P<0,05) – 57% (P<0,001). 2. Magnesium content in pancreatic β cells of mice decreased from 18% (P<0,01) in the case of administration of 8-TSQ to 64% (P<0,001) – alloxan, and in rats – by 15-54% (P<0,001). 3. In islet β-cells, the content of zinc and magnesium in animals decreased from 90 and 82% (P<0,001) – in severe alloxan diabetes to 14 and 23% (P<0,05) – in cases where the disease did not develop. 4. The results of the correlation analysis indicate a significant positive nature of the relationship between zinc and magnesium in pancreatic islets, which indicates a synergistic nature of changes in the content of intracellular metals.
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