Enterococcus bacteria in inflammatory diseases of the oral soft tissues and determination of their sensitivity to antibiotics according to EUCAST

Abstract

The constantly increasing rates of development of antibiotic resistance of microorganisms determine the relevance of implementing the principles of rational antibiotic therapy and unification of methods for studying antibiotic sensitivity according to European protocols. The work carried out microbiological analysis of samples, selected cultures of bacteria of the genus Enterococcus from patients with infectious and inflammatory diseases of the oral cavity and determined the sensitivity of enterococci to antibacterial drugs according to the protocols of EUCAST.The aim of the work was to assess the persistence of enterococci in conditions of inflammatory diseases of the soft tissues of the oral cavity, as well as to determine the level of antibiotic resistance of enterococci according to EUCAST in order to optimize therapeutic approaches.Antibioticogram studies showed that Enterococcus faecium exhibited a high level of sensitivity to fluoroquinolones, vancomycin and a high level of resistance to amoxicillin, and even amoxicillin/clavulanate and amoxicillin/sulbactam. A higher level of sensitivity to antibiotics was characteristic of Enterococcus faecalis, so 67.0% of Enterococcus faecalis and only 47.0% of Enterococcus faecium were sensitive to amoxicillin/clavulanate.The results of the study confirmed the significant prevalence of enterococci in the microbiocenosis of the inflamed area, which indicates their role in the pathogenesis of infectious and inflammatory diseases of the oral cavity. A high frequency of resistance of enterococci to a number of antibiotics was established, which justifies the need for a personalized approach to therapy. The data obtained can be used to improve methods of treatment and prevention of infectious and inflammatory diseases of the oral cavity.It has been shown that the sensitivity assessment of enterococci isolated from the focus of the infectious-inflammatory process of the oral cavity is effective according to the EUCAST criteria, which is associated with the species specificity of genotypic resistance of different species of this genus to antibiotics. The appointment of antimicrobial treatment taking into account the EUCAST criteria makes it impossible to prescribe an ineffective antimicrobial agent.